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KMID : 0360220070480081134
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Journal of the Korean Ophthalmological Society
2007 Volume.48 No. 8 p.1134 ~ p.1142
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Identification of P2Y11 Receptor Expressed in Human Retinoblastoma Cells
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Kim Yoon-Hee
Kim Dae-Ran
Chun Ko-I
Lee Jong-Hyuck
Kong In-Deok
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Abstract
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Purpose: The present study aimed to identify the characteristics and physiological function of the P2Y11 receptor, a receptor likely expressed in human retinoblastoma cells.
Methods: We measured possible P2Y11 signaling in WERI-Rb-1 cells using a Ca2+ imaging technique and RT-PCR.
Results: 1) 10 μM ATP elicited a strong but transient increase in Ca2+ in the WERI-Rb-1 cells, and this Ca2+ rise was well maintained after external Ca2+-depletion. 2) ATP-induced Ca2+ response arose entirely through Ca2+ mobilization. 3) P2Y11 agonist (BzATP, 100 μM) increased Ca2+ by 31.2±3.7 % of ATP effect. 4) mRNA for P2Y11 subtype was identified using RT-PCR.
Conclusions: P2Y11 purinergic activation can increase the intracellular calcium level through calcium mobilization in undifferentiated retinoblastoma cells, which may play an important role in cell proliferation, differentiation, and even pathologic processes.
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KEYWORD
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Calcium, P2Y11 receptor, Retinoblastoma cell
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